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Mark G. Goebl, Ph.D.

goebl_mg.jpg

 

Professor, Graduate Advisor

Department of Biochemistry and Molecular Biology
Indiana University School of Medicine
John D. Van Nuys Medical Science Building
635 Barnhill Drive, Room 4071A
Indianapolis, Indiana 46202-5126

Phone: (317) 274-2055
Facsimile: (317) 274-4686
E-mail: mgoebl@iu.edu 

Goebl Lab Research Site

 

B.S. in Microbiology, 1980, University of Notre Dame, South Bend, IN
Ph. D. in Molecular Genetics and Cell Biology, 1985, The University of Chicago, Chicago, IL
Postdoctoral Fellow, 1986-1989, University of Washington, Seattle, WA

 

  • Read this letter sent to Mark by U.S. Senator G. Richard Lugar...
  • Read this article from Nuvo Magazine about Mark's research... 
  • Watch this video describing the Goebl lab ethanol research...

 

Area of Study

Molecular biology and genetics of cell division cycle regulatory mechanisms in yeast.   More details...

 

Selected Recent Publications

Zhao, W., Breese, E., Bowers, A., Hoggatt, J., Broxmeyer, H.E., Goebl, M., Harrington, M.A.  2013.  SIMPL enhancement of tumor necrosis factor?α dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function.  PLoS ONE 8:e61123.

Adler, J.J., Heller, B.L., Bringman, L.R., Ranahan, W.P., cockling, R.R., Goebl, M.G., Oh, M., Lim, H.S., Igham, R.J., Wells, C.D.  2012.  Amot130 adapts Atrophin?1 Interacting Protein 4 to inhibit Yes-associated protein signaling and cell growth.  J. Biol. Chem. 288:  15181-15193.

Cocklin, R. and Goebl, M.  2011.  Nutrient sensing kinases PKA and Sch9 phosphorylate the catalytic domain of the ubiquitin-conjugating enzyme Cdc34.  PLoS One 6: e27099.

Lass, A., Cocklin, R., Scaglione, K.M., Skowyra, M., Korolev, S., Goebl, M., and Skowyra, D.  2011.  The loop-less tmCdc34 E2 mutant defective polyubiquitination activity in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2.  Cell Div. 6: 7-25.  PMID:21453497.

Cocklin, R., Heyen, J., Larry, T., Tyers, M., and Goebl, M. 2011. New Insight Into the Role of the Cdc34 Ubiquitin-Conjugating Enzyme in Cell Cycle Regulation via Ace2 and Sic1. Genetics 187(3): 701-15. Epub 2010 Dec 31.

Lin, L. Wagner, M.C., Cocklin, R., Kuzma, A., Harrington, M., Molitoris, B.A., Goebl M.G. 2011. The antibiotic gentamicin inhibits specific protein trafficking functions of the Arf1/2 family of GTPases. Antimicrob. Agents Chemother.55(1): 246-54. Epub 2010 Oct 18. PMID:20956596

Benson, E.A., Goebl, M.G., Yang, F.C., Kapur, R., McClintick, J., Sanghani, S., Clapp, D.W., Harrington, M.A. 2010. Loss of SIMPL compromises TNF-alpha-dependent survival of hematopoietic progenitors. Exp. Hematol. 38(2): 71-81. Epub 2009 Nov 23. PMID:19941935

Radivojac, P., Vacic, V., Haynes, C., Cocklin, R.R., Mohan, A., Heyen, J.W., Goebl, M.G., Iakoucheva, L.M. 2010. Identification, analysis, and prediction of protein ubiquitination sites. Proteins 78(2):365-80. PMID:19722269

Lockett, Angelia, Goebl, M. and Harrington, M.A. 2008. Transient membrane recruitment of IRAK-1 in response to LPS and IL-1{beta} requires TNF R1. Am J Physiol Cell Physiol. 295(2):C313-23.

Zhong, Y., Chen, J.Y., Heyen, J., Ott, L.W., Woods, C., Harrington, M.A., and Goebl, M.G. 2007. Data Management in Expression-based Proteomics. Biological Database Modeling. Published in Artech House, pp. 143-162.

Ott, L.W., Resing, K.A., Sizemore, A.W., Heyen, J.W., Cocklin, R.R., Pedrick, N.M., Woods, H.C., Chen, J.Y., Goebl, M.G., Witzmann, F.A. and Harrington, M.A. (2007)  Tumor  Necrosis Factor-α and interleukin-1 induced cellular response:  coupling proteomic and genomic information.  J. Proteomic Sciences 6(6):2176-85.

Luo, Y., Kwon, H-J., Montano, S., Georgiadis, M., Goebl, M. G., and Harrington, M. A. (2007) Phosphorylation of SIMPL modulates RelA associated NF-κB dependent transcription.  Am J Physiol Cell Physiol. 292(3):C1013-23.

Brutkiewicz, S, Mendonca, M., Stantz, K., Comerford, K., Bigsby, R., Hutchins, G., Goebl, M. and Harrington, M. (2007) The expression level of luciferase within tumor cells can alter tumor growth upon in vivo bioluminescence imaging. Luminescence.22:  221-228.

Yan, Zhong, Jake Y. Chen, Josh Heyen, Lee W Ott, Cary Woods, Maureen A Harrington, and Mark G Goebl (2007) Data Management in Expression-based Proteomics, in Database Modeling in Biology: Theories and Practices.  (In press).

Wagner, M.C., Molnar, E.E., Molitoris, B.A., and Goebl, M.G.  2006.  Loss of the homotypic fusion and vacuole protein sorting or Golgi-associated retrograde protein vesicle tethering complexes results in gentamicin sensitivity in the yeast Saccharomyces cerevisiae.  Antimicrob. Agents Chemother. 50:  587-595.

Schweitzer, K., Cocklin, R., Garrett, L., Desai, F., and Goebl, M.  2005.  The ubiquitin ligase SCFGrr1 is necessary for pheromone sensitivity in Saccharomyces cerevisiae.  Yeast 22:  553-564.

Kwon HJ, Breese EH, Vig-Varga E, Luo Y, Lee Y, Goebl MG, Harrington MA. 2004 Tumor necrosis factor alpha induction of NF-kappaB requires the novel coactivator SIMPL.  Mol Cell Biol. 21:9317-26.

Linghu B, Callis J, and Goebl MG.  2002.  Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p.  Euk. Cell 1: 491-494.

Yu KY, Kwon HJ, Norman DAM, Vig E, Goebl MG, and Harrington MA.  2002.  Cutting edge: Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling.  J. Immunol.169: 4075-4078.

Hurteau JA, Allison BM, Brutkiewicz SA, Goebl MG, Heilman DK, Bigsby RM, and Harrington MA.  2001.  Expression and subcellular localization of the cyclin-dependent kinase inhibitor p27(Kip1) in epithelial ovarian cancer.  Gynecol. Oncol. 83: 292-298.

Vig E, Green M, Liu YW, Yu KY, Kwon HJ, Tian J, Goebl MG, and Harrington MA.  2001.  SIMPL is a tumor necrosis factor-specific regulator of nuclear factor-kappa B activity.  J. Biol. Chem. 276: 7859-7866.

Smothers DB, Kozubowski L, Dixon C, Goebl MG, and Mathias N.  2000.  The abundance of Met30p limits SCFMet30p complex activity and is regulated by methionine availability.  Mol. Cell. Biol. 20: 7845-7852.

Mathias N, Johnson S, Byers B, and Goebl M.  1999.  The abundance of cell cycle regulatory protein Cdc4p is controlled by interactions between its F box and Skp1p.  Mol. Cell. Biol. 19: 1759-1767.

 

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Research Interests

This laboratory uses Baker's yeast to study the regulation of early cell cycle events required for the initiation of S phase and DNA replication. We have found that an enzyme responsible for attaching the highly conserved protein ubiquitin onto other protein substrates is necessary for the transition into S phase. Our immediate goals are to determine how this ubiquitin-conjugating activity is regulated as well as to identify its substrate proteins. A variety of molecular, genetic, and immunochemical approaches will be employed.