Supplied Primer
The Sequencing Core provides primers listed below at no additional cost:
| Primer | Abreviation | Sequence (5' to 3') |
| M13 Forward | M13 | TGT AAA ACG ACG GCC AGT |
| M13 Reverse | M13R | CAG GAA ACA GCT ATG ACC |
| T7 Promotor | T7 | TAA TAC GAC TCA CTA TAG GG |
| T7 Terminator | T7 ter | GCT AGT TAT TGC TCA GCG G |
| BGH Reverse | BGHre | TAG AAG GCA CAG TCG AGG |
| T3 | T3 | ATT AAC CCT CAC TAA AGG G |
| SP6 | Sp6 | ATT TAG GTG ACA CTA TAC |
| LUC | Luc | CTT TAT GTT TTT GGC GTC TTC CA |
| PGEX3' | Pgex3' | CCG GGA GCT GCA TGT TCA GAG G |
| PGEX5' | Pgex5' | GGG CTG GCA AGC CAC GTT TGG TG |
| T7Reverse | T7R | TAG TTA TTG CTC AGC GGT GG |
| PCMV Forward | PCMVFwd | CGC AAA TGG GCG GTA GGC GTG |
The following are some tips to help design your custom primers:
· The primer should be at least 15 to 20 bases from the insert.
· The primer should be between 18 to 25 bases long.
· Aim for about 50% GC content, with the Gs and Cs evenly distributed throughout the primer.
· Avoid runs of identical nucleotides.
· The Tm should be 55°C to 75°C.
· Use software to check your primer for any dimers or hair-pin loops that may be formed.
